Microsatellite loci developed for the temperate grasses Anthoxanthum spp. using high-throughput sequencing
A total number of 15 polymorphic loci have been developed by researchers from the Universidade da Coruña in collaboration with AllGenetics.
We are glad to inform that a new primer note in which AllGenetics took part has just come out. The primer note, co-authored by our colleague Dr. Neus Marí-Mena, has been published in the journal Applications in Plant Sciences.
Anthoxanthum-specific polymorphic microsatellite markers have been developed by researchers from the Universidade da Coruña (Spain) and AllGenetics. The researchers prepared and sequenced a microsatellite-enriched library in an Illumina MiSeq. Afterwards, they developed several primer pairs and biologically tested them in individuals belonging to different lineages and populations.
Previous phylogenetic studies had revealed that four diploid and three polyploid lineages conform the Eurosiberian complex of Anthoxanthum. The novel microsatellite loci developed will be used to determine the geographic patterns of gene flow within the different lineages of this grass genus, as well as unravel the origin of its polyploid groups.