DNA barcoding is the leading method for species-level identification and for the authentication of biological samples. Since 2003, it has systematically been applied not only to biological research, but also to several industrial fields.
The method is based on obtaining a DNA sequence from the sample (the DNA barcode). This sequence is then compared to those in reference databases. This fast and cost-effective technique, can even be applied when only a small amount of sample is available.
The DNA barcoding method involves DNA isolation, PCR, sequencing, and the bioinformatic analysis of the DNA sequences generated.
Deliverables include your results, the raw data generated (which will be delivered through our server), and a summary of the methods followed.
Researchers at Aarhus Universitet were interested in DNA barcoding Carcharhinus shark samples from Vietnamese waters. Morphological analyses suggested that two different species existed in the pool of individuals studied. Ethanol-preserved samples were shipped to AllGenetics; DNA isolation, amplification of two mitochondrial regions, sequencing, and bioinformatic analyses were carried out. The mitochondrial sequences were compared with shark reference sequences available in international databases using phylogenetic methods. The sequences obtained from the Vietnamese sharks clustered into two well differentiated groups, each of which corresponded to a different Carcharhinus species.