Experts in DNA barcoding
Species identification by DNA barcoding
DNA barcoding is the leading method for species-level identification and for the authentication of biological samples. This method is based on obtaining a DNA sequence from a specific genomic region of the sample (the DNA barcode). This sequence is then compared to those in reference databases. DNA barcoding is cost-effective and it can even be applied when only a small amount of sample is available.
What you receive
With our standard service, you receive a summary of the methods followed, the raw electropherogram files, your manually-edited sequences in FASTA format, and the results of the species identification. If you require additional analyses, please let us know and we will do our best to meet your needs.
What we need
We can start working by using either your tissue samples, or already extracted DNA.
If you are sending tissue samples, please make sure that they have been properly preserved for DNA isolation (ethanol, silica gel, frozen, or another suitable preservation method). If required, we can provide sampling kits and sample collection guidelines to ensure that your samples arrive at our lab in optimal conditions.
If your DNA has already been extracted, we ideally require 20 μL of DNA in TE buffer or water at a minimum concentration of 10 ng/μL, and 260/280 and 260/230 absorbance ratios higher than 1.8.
Please note that, depending on the specifics of your project, these requirements may vary. Please contact us for the specific requirements of your project.
How we work
Step 1: we isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.
Step 2: we amplify the genomic region of interest (the DNA barcode) using universal or in-house-developed primer pairs.
Step 3: we sequence the PCR products in both directions.
Step 4: we analyse the electropherograms obtained and compare the resulting sequences against reference databases to find the matching species.
For your convenience, we can carry out the entire project or only steps 2, 3, and 4. Please contact us for further information and pricing.