Experts in identifying bacterial, fungal, and algal species by DNA barcoding
16S and 18S-ITS1 sequencing for microbial species registration
According to national guidelines, all microorganisms which are component materials of a fertilising product must be identified and characterised for their inclusion in fertiliser product catalogues.
Identification must be done at the species level, and supported by molecular data obtained by sequencing specific genome regions (the 16S region in bacteria and the 18S-ITS1 region in fungal and algal species).
The DNA barcoding method provides a reliable, rapid, and cost-effective tool for species-level identifications.
How we work
Step 1: We isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.
Step 2: We amplify the genomic region of interest (the DNA barcode) using universal or in-house-developed primer pairs.
Step 3: We sequence the PCR products in both directions.
Step 4: We analyse the electropherograms obtained and compare the resulting sequences against reference databases to find the matching species.
Please contact us for further information and pricing.
What you receive
A species identification certificate.
A report with a summary of the methods followed.
The raw electropherogram files.
Your manually-edited sequences in FASTA format.
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery. We offer up to five 60-min sessions per project, depending on the project size.
What we need
Your cultures appropriately preserved (fresh, frozen, or lyophilised). Please contact us if you need assistance on how to preserve and ship your cultures.