Experts in identifying mixed materials by DNA metabarcoding

Infer the complete list of ingredients in mixed food products

By combining DNA barcoding and high-throughput sequencing, our DNA metabarcoding service enables the simultaneous identification of multiple species present in a complex food matrix, even without a prior knowledge of the expected composition. This is what makes DNA metabarcoding a powerful tool in a number of applications, including:

  • Identification of constituent plant species in herbal products such as tea blends, spices, plant-based medicines, and food supplements.
  • Characterisation of the floral composition of honey.
  • Detection of oil adulteration.
  • Species verification of fruit-based products and vegetal beverages (juices, jams, yoghurt).
  • Identification of animal species in processed meat products.
  • Microbiome profiling of dairy products.
  • Characterisation of wine microorganisms.

Importantly, DNA metabarcoding can be applied to processed material containing highly degraded DNA, such as herbal extracts, oils, powders, or capsules.

Our DNA metabarcoding-based analyses can test the homogeneity of a sample, check if a species of interest is present in a complex mixture, or infer the complete list of ingredients in a sample. Therefore, systematic analysis of batches of products can prevent harmful species substitution and detect unlisted species being used as fillers, thereby ensuring consumer’s confidence and brand protection.

How we work

Identification mixed materials workflow

Step 1: We isolate DNA from different food matrices such as herbal blends, juices, dairy products, etc.

Step 2: We prepare DNA metabarcoding libraries for the target taxonomic group(s) -bacteria, fungi, algae, plants or animals-. For this, we use universal or in-house-developed primer pairs.

Step 3: We sequence the DNA metabarcoding libraries using the Illumina MiSeq technology.

Step 4: We analyse the data obtained in the previous step to obtain the taxonomic composition of the samples.

Please contact us for further information and pricing.

What you receive

A report with a summary of the methods followed.

The raw high-throughput sequencing files.

The results of the bioinformatic analysis including:

  • A list of the Operational Taxonomic Units (OTUs) retrieved per sample.
  • The number of sequence reads assigned to each OTU in each sample.
  • A graphic representation of the relative abundances of the different OTUs in each sample.

If you require additional analyses, please let us know and we will do our best to meet your needs.

At no additional cost, our project specialists will be available to you after project delivery. We offer up to five 60-min sessions per project, depending on the project size.

What we need

Your samples appropriately preserved depending on the sample type (ethanol, silica gel, frozen, or another suitable preservation method). If required, we provide sampling kits and sampling collection guidelines to ensure that your samples arrive at our lab in optimal conditions.

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