Experts in metagenomics
Communities characterisation through whole-genome sequencing
Whole-genome shotgun metagenomics provides complete information about the species composition of an environmental sample, but also offers extensive information about functional diversity and metabolic networks, allowing for gene prediction. This technology detects all the microorganisms present in a given sample, and it is the only method for exploring global viral diversity. It is also possible to carry out RNA-based metatranscriptomic analysis in order to characterise the expression patterns of all active organisms within a community.
Metagenomics and metatranscriptomics can be applied to a variety of fields, such as microbial ecology, marine and freshwater biology, ecosystem monitoring, and host-microbe interactions.
How we work
Step 1: We isolate either DNA or RNA from your samples using tailored isolation protocols.
Step 2: We prepare genomic libraries using the kits recommended by Illumina.
Step 3: We sequence the libraries using the Illumina MiSeq or HiSeq technologies. It is standard to obtain 5 gigabases of raw data per library but we can adapt the output to your specific requirements.
Step 4: We analyse the high-throughput sequencing data to obtain your annotated metagenomes.
For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly. Please contact us for further information and pricing.
What you receive
A report with a summary of the methods followed.
The raw high-throughput sequencing files.
The results of the bioinformatic analysis including demultiplexing, pre-processing and quality control of the raw data, binning, de novo assembly, taxonomic assignment and profiling between samples, and automatic functional annotation
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.
What we need
We can start working with your cultures or environmental samples, which have been appropriately preserved, or your DNA or RNA samples which have already been extracted.
If your DNA has already been extracted, we ideally require 20 μL of DNA in TE buffer or water at a minimum concentration of 50 ng/μL and 260/280 and 260/230 absorbance ratios higher than 1.8 per sample.
If your RNA has already been extracted, we ideally require a minimum amount of 2 μg of high-quality total RNA in nuclease-free water (concentration >40 ng/μL) per sample, as measured by the Agilent 2100 Bioanalyzer or equivalent. RNA should be shipped in dry ice to prevent it from degrading.
Please note that, depending on the specifics of your project, these requirements may vary. Please contact us for the specific requirements of your project.