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Experts in microsatellite genotyping

Let us genotype for you

Have polymorphic microsatellite loci been developed for your study species? If not, please, have a look at our microsatellite development service. If markers have already been developed, we can directly genotype your samples for you.

Microsatellite markers are widely used in population genetics and phylogeography, as well as in kinship analysis. However, the process of genotyping can be tedious and time-consuming. Therefore, we offer a microsatellite genotyping service that will free you from this tiresome task.

What you receive

With our standard service, you receive a summary of the methods followed, the raw electropherogram files, and the results of the bioinformatic analysis including a table with the alleles called at each locus in each individual. If you require additional analyses, please let us know and we will do our best to meet your needs.

What we need

We can start working by using either your tissue samples, or already extracted DNA.

If you are sending tissue samples, please make sure that they have been properly preserved for DNA isolation (ethanol, silica gel, frozen, or another suitable preservation method). If required, we can provide sampling kits and sample collection guidelines to ensure that your samples arrive at our lab in optimal conditions.

If your DNA has already been extracted, we ideally require 50 μL of DNA in TE buffer or water at a minimum concentration of 10 ng/μL, and 260/280 and 260/230 absorbance ratios higher than 1.8.

Please note that, depending on the specifics of your project, these requirements may vary. Please contact us for the specific requirements of your project.

How we work

Step 1: we isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.

Step 2: we amplify the microsatellite loci under study using fluorescently-labelled primer pairs.

Step 3: we run the labelled amplicons in the sequencer.

Step 4: we analyse the electropherograms generated and call the alleles observed at each locus.

 

For your convenience, we can carry out the entire project or only steps 2, 3 and 4. Please contact us for further information and pricing.

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