Experts in Genotyping

Microsatellite markers are widely used in population genetics and phylogeography projects, as well as in kinship analysis. However, the process of genotyping can be tedious and time-consuming. Therefore, at AllGenetics we offer a microsatellite genotyping service that will free you from this tiresome task. This service will enable you to dedicate more time to the important aspects of your project.

Let us genotype for you

Have polymorphic microsatellite loci been developed for your study species? If not, please, have a look at our microsatellite development service. If markers have already been developed, we can directly genotype your samples for you.

How we work

There are 3 steps in our microsatellite genotyping service. For your convenience, we can carry out the entire project or only the parts you need.

  • In Step 1 we isolate DNA from samples appropriately preserved (ethanol, RNAlater, silica-gel dried, or any other suitable preservation method).  We have adapted different DNA isolation protocols, depending on the starting biological material. If Step 1 is not ordered, we would need high quality DNA at a concentration of, at least, 20 ng/μL from the number of individuals you wish to genotype.
  • In Step 2 we carry out the PCRs using fluorescently-labelled primers, and run the amplicons in the sequencer.
  • In Step 3 we analyse the electropherograms generated in Step 2 and call the alleles observed at each locus.

Deliverables include your results, the raw data generated (which will be delivered through our server), and a summary of the methods followed.

Genotyping daisies

We were asked by researchers at the Real Jardín Botánico - CSIC (Madrid) to genotype hundreds of daisies using pre-developed microsatellite markers. Our technical staff carried out a pilot project using a reduced number of individuals. In this pilot project we tested the performance of the primers provided by the clients, the quality of the PCRs, and the quality of the electropherograms. Once the experiments were optimised, we genotyped the remaining samples. The electropherograms were delivered to the researchers, along with pictures of all the PCR gels produced.