DNA sequencing has become essential for all branches of biological research. However, until recently, the existing sequencing techniques had certain limitations that greatly diminished the full potential that, virtually, they could achieve. Next-generation sequencing is capable of overcoming these problems and produce great amounts of data in very little time.
Classic sequencing techniques are still really useful when dealing with few, small genome regions but have more difficulties when higher throughput, speed, and scalability are needed.
Next-generation sequencing encompasses cost-effective techniques which allow researchers to quickly obtain massive amounts of high-quality sequencing data. Once the DNA is isolated from your samples, genomic libraries are constructed according to the project goals. Libraries corresponding to different samples can be sequenced at once (multiplexed) due to sample-specific oligonucleotide tags or indices which are added during the construction of the genomic libraries.
At AllGenetics we use the Illumina (HiSeq and MiSeq) and PacBio technologies to construct and sequence different types of genomic libraries. Our pipelines will enable you to sequence:
Our projects are divided into 4 steps. For your convenience, we can carry out the entire project or only the parts you need.
Researchers from a Spanish research centre shipped multiple, already-amplified mitochondrial genomes to AllGenetics. In our lab, we constructed the amplicon libraries using the Illumina's Nextera XT kit. Then, all the libraries were pooled together in equimolar amounts and sequenced in a lane of the HiSeq 2000 platform with the PE100 chemistry. Deliverables included comprehensive methodological reports and the raw sequencing data, which were delivered to the client through our web server.