Experts in Next-Generation Sequencing

DNA sequencing has become essential for all branches of biological research. However, until recently, the existing sequencing techniques had certain limitations that greatly diminished the full potential that, virtually, they could achieve. Next-generation sequencing is capable of overcoming these problems and produce great amounts of data in very little time.

High-throughput genome sequencing

Classic sequencing techniques are still really useful when dealing with few, small genome regions but have more difficulties when higher throughput, speed, and scalability are needed.

Next-generation sequencing encompasses cost-effective techniques which allow researchers to quickly obtain massive amounts of high-quality sequencing data. Once the DNA is isolated from your samples, genomic libraries are constructed according to the project goals. Libraries corresponding to different samples can be sequenced at once (multiplexed) due to sample-specific oligonucleotide tags or indices which are added during the construction of the genomic libraries.

At AllGenetics we use the Illumina (HiSeq and MiSeq) and PacBio technologies to construct and sequence different types of genomic libraries. Our pipelines will enable you to sequence:

  • Microbial genomes.
  • Mitochondrial and plastid genomes.
  • Eukaryotic nuclear genomes (including DNA methylome profiling).
  • PCR-amplified genomic regions.
  • Custom target-enriched libraries.

How we work

Our projects are divided into 4 steps. For your convenience, we can carry out the entire project or only the parts you need.

  • In Step 1 we isolate DNA from samples appropriately preserved (ethanol, RNAlater, silica-gel dried, or any other suitable preservation method). We have adapted different DNA isolation protocols, depending on the starting biological material.
  • In Step 2 we construct the genomic libraries according to the project goals. Different factors should be considered at this point: for example, the amount of input DNA available, which can vary among sample types, the size of the target genome, the level of coverage required, or the GC content of the sample.
  • In Step 3 we sequence the libraries using the Illumina (HiSeq and MiSeq) and PacBio technologies. Millions of reads per library are obtained in this step.
  • In Step 4 we carry out on-demand, high-throughput bioinformatic analyses. Our data analysis services enable a broad range of DNA-seq applications, including:
    • De novo and reference-guided genome assembly.
    • Genome annotation.
    • Single nucleotide polymorphism (SNP) detection and analysis.
Deliverables include your results, the raw data generated (which will be delivered through our server), and a summary of the methods followed.

Studying mitochondrial genomes using next-generation sequencing

Researchers from a Spanish research centre shipped multiple, already-amplified mitochondrial genomes to AllGenetics. In our lab, we constructed the amplicon libraries using the Illumina's Nextera XT kit. Then, all the libraries were pooled together in equimolar amounts and sequenced in a lane of the HiSeq 2000 platform with the PE100 chemistry. Deliverables included comprehensive methodological reports and the raw sequencing data, which were delivered to the client through our web server.