Using DNA metabarcoding to characterise the arbuscular mycorrhizal fungi in roots of native and invasive plants

Dr. Antonio Roldán

CSIC

“Our lab has carried out several projects (bacteria, fungi, and AM fungi) with the invaluable assistance of AllGenetics. This collaboration has yielded excellent publications and positive feedback. They are always willing to solve any doubts or requests. Consequently, I strongly recommend AllGenetics services… You will never be disappointed”

GOAL

The research team wanted to compare the arbuscular mycorrhizal fungi (AMF) communities hosted by the invasive plant Pennisetum setaceum with those from the coexisting native plant Hyparrhenia hirta at different semiarid locations with different edaphic characteristics.

APPROACH

DNA was isolated from the thinner roots of each plant sample using a commercial kit. A fragment of the 18S rRNA gene, typically used to characterise AMF species, was amplified during DNA metabarcoding library preparation, and sequenced. We pre-processed and analysed the MiSeq reads and used the MaarjAM database as a reference to obtain the species composition of each sample.

RESULTS

The researchers found that the AMF species detected in the native and invasive species were not plant-specific but rather location-specific. They concluded that P. setaceum is able to associate with local AMF communities outside its native range.

This research has been published in the journal Science of the Total Environment.

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