High-throughput DNA metabarcoding of feather mites

Dr. Jorge Doña

University of Illinois

"Read no more and prepare your samples! If you are interested in outsourcing your genomic project, AllGenetics is probably your best option. AllGenetics has an enormous capacity to carry out any kind of projects; from studying the diet of tiny mites using DNA metabarcoding to de-novo assembling a large plant genome. The team is composed of highly qualified professionals, and the overall quality standard is very high. Last but not least, you never feel you are on your own at any stage of the project, as they are always willing to help"

GOAL

The research team was interested in developing a high-throughput method to identify to the species level hundreds of mite infracommunities.

APPROACH

At AllGenetics, we extracted DNA from over 3000 samples using a non-destructive protocol. DNA metabarcoding libraries were prepared by amplifying a fragment of the COI mitochondrial region, and sequenced in the Illumina MiSeq platform. The sequencing results were analysed and the taxonomy was assigned using an extensive DNA barcoding database previously developed by the research team.

RESULTS

By studying the DNA metabarcoding results generated by AllGenetics in detail, the research team was able to unveil unexpected combinations of birds and feather mites, with important evolutionary implications.

This research has been published in the journals Molecular Ecology and Experimental and Applied Acarology.

Learn more about DNA metabarcoding Case studies