How to explore bacterial diversity using next-generation sequencing?
Massive sequencing of bacterial 16S rRNA can be considered as a milestone in Microbiology. Taking advantage of a variety of phylogenetic methods, nucleotide variation at this genomic region is widely used to classify bacterial samples into already known or even into new species and genera.
In recent years, next-generation sequencing technologies have revolutionised the field of Microbiology since they have prompted the identification of uncultured microorganisms from almost any ecosystem. Considering that the vast majority of microbial species do not grow in the lab, these new technologies open up huge possibilities.
Such studies, that are often referred to as bacterial metagenomics, are indeed a hot topic in several areas of life science. For instance, using metagenomics, we can determine the bacterial community associated to a particular human organ, or compare the bacterial assemblages that live in different types of soil.
At this INFUSCIENCE, Margarita Poza will present her pioneering work on bacterial diversity in hospital environments that has been recently published in PLoS ONE. In this paper, Margarita and colleagues compared the microbiota detected at the intensive care unit of a hospital to that found at the hospital's main entrance.
Margarita Poza obtained her PhD thesis on microbial biotechnology at the University of Santiago de Compostela in 2002. After several research internships abroad, one of them at Georgetown University (USA), she joined the Biomedical Research Institute (INIBIC A Coruña) as a tenure-track scientist. At INIBIC, Margarita has been studying the molecular mechanisms of bacterial resistance, including the expression of biofilm-related genes in the human pathogen Acinetobacter baumannii. Various projects in transcriptomics that were carried out using next-generation sequencing lead her to the new field of metagenomics.
December 21st 2012 | 17 pm | Ed. de Servicios Centrales de Investigación. Campus de Elviña s/n. E-15071 A Coruña