Experts in characterising plant clones by microsatellite genotyping
Identify your plant clone with microsatellite markers
For over the past two decades, microsatellites or single sequence repeats (SSRs) have been the most widely used molecular markers in plant genetic studies. In the particular case of cultivated plants, microsatellites have been extensively used for cultivar or varietal DNA fingerprinting, as recommended by the International Union for the Protection of New Varieties of Plants (UPOV 2010, 2011).
Polymorphic microsatellite markers have already been developed for a wide range of model and non-model species. If this is the case for your study species, at AllGenetics we can directly genotype your samples for you. Alternatively, if microsatellite markers are not yet available for your species of interest we can develop them.
How we work
Step 1: We isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.
Step 2: We amplify the microsatellite loci under study using fluorescently-labelled primer pairs.
Step 3: We run the labelled amplicons in the sequencer.
Step 4: We analyse the electropherograms and call the alleles observed at each locus.
Please contact us for further information and pricing.
What you receive
A report with a summary of the methods followed.
The raw electropherogram files.
The results of the bioinformatic analysis including a table with the alleles called at each locus in each individual.
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery. We offer up to five 60-min sessions per project, depending on the project size.
What we need
Your tissue samples appropriately preserved depending on the sample type (ethanol, silica gel, frozen, or another suitable preservation method). If required, we provide sampling kits and sampling collection guidelines to ensure that your samples arrive at our lab in optimal conditions.