Experts in detecting agronomically important genes by RNA-seq
Learn how your plants respond to environmental stressors
There are several molecular methods to explore plant response to specific conditions. Among all the methodologies based on gene expression, RNA-seq offers a powerful, cost-effective tool for global gene expression analysis. Screening for genes differentially expressed in a given condition, such as exposure to a biotic or abiotic stressor, can provide extremely useful information which can be used in plant genetic improvement programmes.
At AllGenetics, we analyse the high-throughput sequencing data obtained from the plant transcriptome in order to identify candidate genes underlying phenotypic traits of interest.
How we work
Step 1: We isolate RNA from your plant samples.
Step 2: We purify the RNA fraction you are interested in (total RNA, mRNA, small RNAs...) and prepare cDNA libraries using the kits recommended by Illumina.
Step 3: We sequence the libraries using the Illumina HiSeq or NovaSeq technologies. Millions of reads per library are obtained in this step.
Step 4: We analyse the high-throughput sequencing data obtained in the previous step.
What you receive
A report with a summary of the methods followed.
The raw high-throughput sequencing files.
The results of the bioinformatic analyses including:
- Gene expression quantification.
- Detection of differential gene expression between conditions.
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery. We offer up to five 60-min sessions per project, depending on the project size.
What we need
Your plant tissue samples which have been appropriately preserved for RNA isolation. We provide sampling kits and sampling collection guidelines to ensure that your samples arrive at our lab in optimal conditions.