Experts in identifying genes related to traits of interest by RNA-seq
Learn how your grapevines respond to environmental stressors
RNA-seq is a powerful, cost-effective tool for surveying plant response to specific conditions. Knowing which genes are being expressed under certain circumstances can help you select grapevine varieties or cultivars more resistant to stressors such as drought, frost, or pathogens.
At AllGenetics we use high-throughput sequencing to identify candidate genes underlying phenotypic traits of interest, providing invaluable information for genetic improvement programs.
How we work
Step 1: We isolate RNA from your samples. We have adapted different RNA isolation protocols depending on the starting biological material.
Step 2: We purify the RNA fraction you are interested in (total RNA, mRNA, small RNAs...) and prepare cDNA libraries using the kits recommended by Illumina.
Step 3: We sequence the libraries using the Illumina HiSeq technology. Millions of reads per library are obtained in this step.
Step 4: We analyse the high-throughput sequencing data obtained in the previous step.
Please contact us for further information and pricing.
What you receive
A report with a summary of the methods followed.
The raw high-throughput sequencing files.
The results of the bioinformatic analyses including:
- Gene expression quantification.
- Detection of differential gene expression between conditions.
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery. We offer up to five 60-min sessions per project, depending on the project size.
What we need
Your plant tissue samples which have been appropriately preserved for RNA isolation. We provide sampling kits and sampling collection guidelines to ensure that your samples arrive at our lab in optimal conditions.