Experts in identifying grapevine varieties by microsatellite markers
Identify your plant clone with microsatellite markers
For over the past two decades, microsatellites or simple sequence repeats (SSRs) have been the most widely used molecular markers in plant genetic studies. In the particular case of grapevines, microsatellites have been extensively used for cultivar or varietal DNA fingerprinting, as recommended by the International Union for the Protection of New Varieties of Plants (UPOV 2010, 2011).
At AllGenetics we can genotype your samples using the following internationally accepted loci: VVS2, VVMD5, VVMD7, VVMD25, VVMD27, VVMD28, VVMD32, VrZAG62, and VrZAG79. This analysis will allow you to identify and verify your varieties and cultivars for breeding programs or legal protection. They will also serve as a crossreference for authorised varieties.
How we work
Step 1: We isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.
Step 2: We amplify the microsatellite loci under study using fluorescently-labelled primer pairs.
Step 3: We run the labelled amplicons in the sequencer.
Step 4: We analyse the electropherograms and call the alleles observed at each locus.
Please contact us for further information and pricing.
What you receive
A report with a summary of the methods followed.
The raw electropherogram files.
The results of the bioinformatic analysis including a table with the alleles called at each locus in each individual.
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery. We offer up to five 60-min sessions per project, depending on the project size.
What we need
Your tissue samples which have been appropriately preserved (ethanol, silica gel, frozen, or another suitable preservation method). If required, we provide sampling kits and sampling collection guidelines to ensure that your samples arrive at our lab in optimal conditions.