Experts in bacterial genome sequencing, assembly, and annotation
Characterise your bacterial strains through whole-genome sequencing
Characterising bacterial genomes through whole-genome sequencing is vitally important in the fields of medicine, ecology, and biotechnology. This is why our service is more and more in demand by microbiologists from different research backgrounds and with divergent goals.
At AllGenetics, we use high-throughput sequencing to obtain the annotated genomes of your bacterial strains. To do so we take advantage of Illumina’s MiSeq technology which enables us to obtain the genomes cost-effectively and accurately.
Please note that repetitive genomic regions may be difficult or impossible to assemble using Illumina's short reads. In this case, Illumina's MiSeq sequencing can be complemented with Oxford Nanopore’s MinION or PacBio’s RSII. These are new technologies which provide longer reads that improve genome assembly.
How we work
Step 1: We isolate DNA from your samples using specific DNA isolation protocols for bacterial cells.
Step 2: We prepare genomic libraries using the kits recommended by Illumina.
Step 3: We sequence the libraries using the Illumina MiSeq technology. As a norm, we obtain 500 megabases of raw data per library but we can adapt the output to the genome size of the species under study.
Step 4: We analyse the high-throughput sequencing data to obtain your annotated genomes.
Please contact us for further information and pricing.
What you receive
A report with a summary of the methods followed.
The raw high-throughput sequencing files.
The results of the bioinformatic analysis including demultiplexing, pre-processing and quality control of the raw data, de novo assembly, deduplication of clonal reads, automatic gene annotation, and average nucleotide identity between the genomes sequenced.
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.
What we need
We can start working with either your bacterial cultures which have been appropriately preserved (fresh, frozen, or lyophilised) or your DNA samples which have already been extracted.
If your DNA has already been extracted, we ideally require 20 μL of DNA in TE buffer or water at a minimum concentration of 20 ng/μL and 260/280 and 260/230 absorbance ratios higher than 1.8 per sample.
Please note that, depending on the specifics of your project, these requirements may vary. Please contact us for the specific requirements of your project.