Experts in eukaryotic genome sequencing, assembly, and annotation
Obtain a draft genome of your study species
Obtaining a draft genome of your protist, fungi, animal, or plant under study is of great utility in several research fields. For example, in comparative genomics, when regions up- and downstream the genes of interest need to be characterised; in RNA-seq, where an annotated reference genome is required for read mapping; or in phylogenomics, in which novel genome regions including mitochondrial and chloroplast genomes can be sequenced.
At AllGenetics, we use Illumina's HiSeq technology, which provides millions of high-quality short reads. These reads can be combined with Oxford Nanopore’s MinION or PacBio’s RSII. These are new technologies which provide longer reads that improve genome assembly.
How we work
Step 1: We isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.
Step 2: We prepare genomic libraries using the kits recommended by Illumina.
Step 3: We sequence the libraries using the Illumina HiSeq technology. As a norm, we obtain 50 or 100 gigabases of raw data per library but we can adapt the output to the genome size of the species under study.
Step 4: We analyse the high-throughput sequencing data to obtain your annotated contigs.
Please contact us for further information and pricing.
What you receive
A report with a summary of the methods followed.
The raw high-throughput sequencing files, which will be delivered through our server.
The results of the bioinformatic analysis including demultiplexing, pre-processing and quality control of the raw data, de novo assembly, removal of duplicate reads, automatic gene annotation, and average nucleotide identity between the genomes sequenced. Along with a draft of the nuclear genome, you will also receive the mitochondrial and plastid genomes.
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.
What we need
We can start working by using either your tissue samples, or DNA which has already been extracted.
If you are sending tissue samples, please make sure that they have been properly preserved for DNA isolation (ethanol, silica gel, frozen, or another suitable preservation method). If required, we can provide sampling kits and sample collection guidelines to ensure that your samples arrive at our lab in optimal conditions.
If your DNA has already been extracted, we ideally require 20 μL of DNA in TE buffer or water at a minimum concentration of 50 ng/μL, and 260/280 and 260/230 absorbance ratios higher than 1.8.
Please note that, depending on the specifics of your project, these requirements may vary. Please contact us for the specific requirements of your project.