Experts in targeted capture sequencing
Reduce sequencing costs with our targeted capture sequencing service
Targeted capture or target enrichment allows for simultaneous sequencing of sets of genomic regions of interest in several individuals in the same sequencing run, therefore reducing sequencing costs. This technique uses custom RNA probes which are complementary to the target genomic regions. Our targeted capture sequencing service is widely used by research teams working on phylogenomics.
Step 1: We isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.
Step 2: We prepare genomic libraries compatible with Illumina’s HiSeq and MiSeq platforms. Then, we capture the target genomic regions using MYbaits target enrichment (MYcroarray) or SureSelect target enrichment (Agilent Technologies).
Step 3: We sequence the libraries in Illumina’s HiSeq, NovaSeq, or MiSeq. Thanks to the target enrichment, several libraries can be multiplexed in the same sequencing run and still get a high coverage of the genome regions under study.
Step 4: We analyse the high-throughput sequencing data and obtain the regions of interest for each of the samples analysed.
For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.
What you receive
A report with a summary of the methods followed.
The raw high-throughput sequencing files, which will be delivered through our server.
The results of the bioinformatic analysis including demultiplexing, pre-processing and quality control of the raw data, de novo or reference-guided assembly, removal of duplicate reads, and annotation of the genomic regions of interest.
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.