Experts in targeted capture sequencing
Reduce sequencing costs with targeted capture
Targeted capture or target enrichment allows for simultaneous sequencing of sets of genomic regions of interest in several individuals in the same sequencing run, therefore reducing sequencing costs. This technique uses custom RNA probes which are complementary to the target genomic regions. Genomic libraries are enriched in the target genomic regions and thus the sequencing coverage is kept high.
Targeted capture sequencing is widely used in phylogenomics.
How we work
Step 1: We isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.
Step 2: We prepare genomic libraries compatible with Illumina’s HiSeq and MiSeq platforms. Then, we capture the target genomic regions using MYbaits target enrichment (MYcroarray) or SureSelect target enrichment (Agilent Technologies).
Step 3: We sequence the libraries in Illumina’s HiSeq or MiSeq. Thanks to the target enrichment, several libraries can be multiplexed in the same sequencing run and still get a high coverage of the genome regions under study.
Step 4: We analyse the high-throughput sequencing data and obtain the regions of interest for each of the samples analysed.
For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly. Please contact us for further information and pricing.
What you receive
A report with a summary of the methods followed.
The raw high-throughput sequencing files, which will be delivered through our server.
The results of the bioinformatic analysis including demultiplexing, pre-processing and quality control of the raw data, de novo or reference-guided assembly, removal of duplicate reads, and annotation of the genomic regions of interest.
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.
What we need
We can start working by using either your tissue samples, or DNA which has already been extracted
If you are sending tissue samples, please make sure that they have been properly preserved for DNA isolation (ethanol, silica gel, frozen, or another suitable preservation method). If required, we can provide sampling kits and sample collection guidelines to ensure that your samples arrive at our lab in optimal conditions.
If your DNA has already been extracted, we ideally require 400 ng of DNA in TE buffer or water at a minimum concentration of 10 ng/μL, and 260/280 and 260/230 absorbance ratios higher than 1.8.
Please note that, depending on the specifics of your project, these requirements may vary. Please contact us for the specific requirements of your project.