Experts in RNA-seq
Differential expression analysis with AllGenetics' RNA-seq service
AllGenetics' RNA-seq service allows us to obtain a large number of reads from the transcriptome which are further analysed using high-throughput bioinformatic methods. The most common application of RNA-seq is to detect differentially expressed genes under given environmental stressors.
Step 1: We isolate RNA from your samples. We have adapted different RNA isolation protocols depending on the starting biological material.
Step 2: We purify the RNA fraction you are interested in (total RNA, mRNA, small RNAs, noncoding RNAs, circular RNAs) and prepare cDNA libraries using the kits recommended by Illumina.
Step 3: We sequence the libraries using the Illumina HiSeq or NovaSeq technologies. Millions of reads per library are obtained in this step.
Step 4: We analyse the high-throughput sequencing data obtained in the previous step.
For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.
What you receive
A report with a summary of the methods followed.
The raw high-throughput sequencing files, which will be delivered through our server.
The results of the bioinformatic analyses including demultiplexing, pre-processing and quality control of the raw data, read alignment against the reference genome, gene expression quantification and detection of differential gene expression among conditions.
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.