High-throughput sequencing using Illumina, PacBio, and ONT: key concepts and applications

Course details

High-throughput sequencing has already revolutionised our understanding of biology and has greatly accelerated research in many areas of the life sciences. In this 11-hour course, participants will be given the tools necessary to understand how this cutting-edge technique works, and how it can be applied to their current or future research.

Participants will learn the basics of the different sequencing chemistries and platforms that are now available in the market with a focus on the Illumina technology. The course will also teach some of the main applications in the domain of life science research, such as RNA-seq, SNP discovery and genotyping, amplicon sequencing, whole genome sequencing, etc., covering the most important aspects of a high-throughput sequencing experiment, from DNA and RNA extraction to library preparation and sequencing.

Language: English or Spanish.

Number of places available: 18.

Instructor: Antón Vizcaíno.

Target audience and requirements

Master students, early career researchers (pre-docs and post-docs), or senior researchers, either from Industry or Academia, with zero or little prior contact with high-throughput sequencing technologies. Participants will be expected to be familiar with the most common molecular biology techniques.

Although this course does not include any data analysis, participants are welcome to bring their own laptops.

Course program

10:00 - 11:30 Introduction to nucleic acid sequencing: from Sanger to Nanopore RNAseq: experimental design, library preparation, and applications
11:30 - 12:00 Coffee! Coffee!
12:00 - 13:30 Short-read sequencing technology: Illumina Amplicon sequencing: experimental design, library preparation, and applications
13:30 - 15:00 Lunch Lunch
15:00 - 16:30 Long-read sequencing technology: Pacific Biosciences and Oxford Nanopore Technologies Whole genome sequencing and bisulphite sequencing: experimental design, library preparation, and applications
16:30 - 17:00 Coffee! Coffee!
17:00 - 18:00 Sample indexing and multiplexing strategies. Index swapping Low-representation library sequencing: experimental design, library preparation, and applications
18:00 - 20:00 Free time Good bye!
20:00 - 22:00 Dinner and party



First edition 2019 (Spanish): March 14 - March 15.

Second edition 2019 (English): September 19 - September 20.


The course will be held at Camping Los Manzanos. Los Manzanos is a singular and quaint place in the municipality of Oleiros (A Coruña, Galicia, Spain). It is located within walking distance of the picturesque XVI-century castle and the lively cafés in the village of Santa Cruz. Travellers who want to enjoy being near the sea, but not right on the sea itself, flock to this original campsite in the woods which is well-known for its restaurant and its sculptures and paintings by Julio Sanjurjo. A Coruña, one of the most beautiful cities in Northern Spain is only 12 km. away.

The easiest way to reach the venue from A Coruña airport is by taxi; alternatively, you may take the airport bus to A Coruña Bus Station and then take the bus line to Santa Cruz. Please do not hesitate to let us know if you need further information on how to get to Los Manzanos.


Course fee (including course materials and refreshments): 350 euros.

Course fee + accommodation and meals: 460 euros. This package includes accommodation in shared bungalows (2 or 4 people) with en suite bathroom (check in on Wednesday evening; check out on Friday morning) and meals (breakfast, lunch, and dinner) at the venue restaurant. The bungalows are equipped with bed linen and towels, TV, coffee maker, toaster, microwave oven, fridge, flatware, dishware, and heating. Additional accommodation options are available at an extra cost.

How to apply

If interested, please fill out our contact form and specify:

  • The course and edition you are interested in enrolling in.
  • The details of the paying institution (including VAT number).

The sign-up deadline is 30 days prior to the start of the course.

Please note that there is an 18 participant limit and applications will be accepted on a first-come, first-served basis.

A 10 participant minimum per course edition is required. If that minimum is not reached the edition will be cancelled and participants offered a different edition or a total refund.