Introduction to high-throughput sequencing: Illumina, Ion Torrent, PacBio, and Oxford Nanopore technologies
High-throughput sequencing has already revolutionised our understanding of biology and has greatly accelerated research in many areas of the life sciences. In this 7-hour course, participants will be given the tools necessary to understand how this cutting-edge technique works, and how it can be applied to their current or future research.
Participants will learn the basics of the different sequencing chemistries and platforms that are now available in the market with a focus on the Illumina technology. The course will also teach some of the main applications in the domain of life science research, covering the main details of a high-throughput sequencing experiment, and focusing on the technical aspects of DNA or RNA isolation, library preparation, and sequencing.
Language: English or Spanish.
Number of places available: 24.
Instructor: Antón Vizcaíno.
Target audience and requirements
Master students, early career researchers (pre-docs and post-docs), or senior researchers, either from Industry or Academia, with zero or little prior contact with high-throughput sequencing technologies. Participants will be expected to be familiar with the most common molecular biology techniques.
Although this course does not include any data analysis, participants are welcome to bring their own laptops.