Characterisation of new microsatellite markers in the viperine snake Natrix maura
Hugo Le Chevalier
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Researchers from CNRS were interested in outsourcing the development of microsatellite markers for Natrix maura, with the goal of using them to identify fine scale genetic structures.
We prepared a genome library enriched in di-, tri-, and tetranucleotide microsatellites. As the starting material, we used DNA obtained from a buccal swab from a Natrix maura individual. The library was sequenced in the Illumina MiSeq platform and, by using in-housed developed scripts, we selected the reads containing microsatellite motifs. Then, we designed primer pairs at the flanking regions of the microsatellites. A total number of 108 primer pairs were tested in 7 individuals.
We obtained 19 polymorphic microsatellite loci that were further tested by the research team for deviation from Hardy-Weinberg equilibrium and linkage disequilibrium in 120 individuals resulting in a selection of 14 high quality polymorphic markers. This research has been published in the journal Ecology and Evolution.