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Experts in eukaryotic genome sequencing

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Obtain a draft genome of your study species with our eukaryotic genome sequencing service

Obtaining a draft genome of your protist, fungi, animal, or plant under study is of great utility in several research fields. For example, in comparative genomics, when we need to characterise regions up- and downstream the genes of interest. In RNA-seq, where we need an annotated reference genome for read mapping. Or in phylogenomics, in which novel genome regions including mitochondrial and chloroplast genomes can be sequenced.

Our lab and bioinformatics teams will work together to offer you the most suitable workflow to get the genome of your study species. Our expertise encompasses high-quality DNA extraction protocols, both short-read and long-read sequencing, and assembly of difficult, large genomes.

This graph shows the different steps of the AllGenetics' eukaryotic sequencing service. Therefore it shows a typical workflow of a eukaryotic genome sequencing experiment, including DNA isolation, library preparation, high-throughput sequencing, and bioinformatic analysis.

Step 1

We isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.

Step 2

We prepare genomic libraries using the kits recommended by Illumina.

Step 3

We sequence the libraries using the Illumina NovaSeq technology. As a norm, we obtain 50 or 100 gigabases of raw data per library but we can adapt the output to the genome size of the species under study.

Step 4

We analyse the high-throughput sequencing data to obtain your annotated contigs.

What you receive

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A report with a summary of the methods followed.
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The raw high-throughput sequencing files, which will be delivered through our server, and the corresponding quality control report including demultiplexing and pre-processing.

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The results of the bioinformatic analysis including de novo assembly, quality control of the assembly, identification of repetitive elements, and automated gene prediction and annotation against curated databases. Along with the draft nuclear genome, you will receive the mitochondria and plastid draft genomes. In the case of fungal genomes we also deliver the results of the mean genomic identity between the sequenced genomes. If you require additional analyses, please let us know and we will do our best to meet your needs.
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At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.

Contact us for further information and pricing

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