Experts in targeted capture sequencing

Reduce sequencing costs with our targeted capture sequencing service

AllGenetics' targeted capture service (also known as target enrichment) allows for the simultaneous sequencing of sets of genomic regions in several individuals. Since the libraries are enriched in the genomic regions of interest, we sequence them in the same sequencing run. This allows us to reduce sequencing costs.

Targeted capture sequencing uses custom RNA probes which are complementary to the target genomic regions.


At AllGenetics we do also offer the option of combining target enrichment and genome skimming. This method is known as Hyb-seq and allows for the recovery of target low-copy regions, as well as off-target high-copy nuclear and plastid regions.

Both the classic targeted capture and Hyb-seq approaches are widely used by research teams focused on phylogenomics.

This graph shows the different steps of the AllGenetics' targeted capture sequencing service. Therefore it shows a typical workflow of a targeted capture sequencing experiment, including DNA isolation, capture, library preparation, high-throughput sequencing, and bioinformatic analysis.

Step 1

We isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.

Step 2

We prepare genomic libraries compatible with Illumina’s NovaSeq and MiSeq platforms. Then, we capture the target genomic regions using MYbaits target enrichment (MYcroarray) or SureSelect target enrichment (Agilent Technologies).

Step 3

We sequence the libraries in Illumina’s NovaSeq or MiSeq. Thanks to the target enrichment, several libraries can be multiplexed in the same sequencing run and still get a high coverage of the genome regions under study.

Step 4

We analyse the high-throughput sequencing data and obtain the regions of interest for each of the samples analysed.

For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.

What you receive

A report with a summary of the methods followed.

The raw high-throughput sequencing files, which will be delivered through our server, and the corresponding quality control report including demultiplexing and pre-processing.

The results of the bioinformatic analysis including mapping to the reference sequences, annotation of the genomic regions of interest, and generation of consensus sequences. If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.

Contact us for further information and pricing