Experts in targeted capture sequencing
Reduce sequencing costs with our targeted capture sequencing service
AllGenetics' targeted capture service (also known as target enrichment) allows for the simultaneous sequencing of sets of genomic regions in several individuals. Since the libraries are enriched in the genomic regions of interest, we sequence them in the same sequencing run. This allows us to reduce sequencing costs.
Targeted capture sequencing uses custom RNA probes which are complementary to the target genomic regions.
At AllGenetics we do also offer the option of combining target enrichment and genome skimming. This method is known as Hyb-seq and allows for the recovery of target low-copy regions, as well as off-target high-copy nuclear and plastid regions.
Both the classic targeted capture and Hyb-seq approaches are widely used by research teams focused on phylogenomics.
Step 1
We isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.
Step 2
We prepare genomic libraries compatible with Illumina’s NovaSeq and MiSeq platforms. Then, we capture the target genomic regions using MYbaits target enrichment (MYcroarray) or SureSelect target enrichment (Agilent Technologies).
Step 3
We sequence the libraries in Illumina’s NovaSeq or MiSeq. Thanks to the target enrichment, several libraries can be multiplexed in the same sequencing run and still get a high coverage of the genome regions under study.
Step 4
We analyse the high-throughput sequencing data and obtain the regions of interest for each of the samples analysed.
For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.
What you receive
The raw high-throughput sequencing files, which will be delivered through our server, and the corresponding quality control report including demultiplexing and pre-processing.
