Experts in amplicon sequencing

Analyse all the gene variants with our amplicon sequencing service

At AllGenetics, we can sequence all the gene variants of heterogeneous multi-copy regions. With our amplicon sequencing service you will be able to recover all the diversity regardless of the number of indels or point mutations. This is extremely useful when studying gene families such as the vertebrate major histocompatibility complex (MHC) or the ribosomal non-transcribed spacers (ITS1 and ITS2).

We run most of our amplicon sequencing projects in Illumina's sequencers such as MiSeq and NovaSeq, as the reads obtained are usually long enough to characterise the genome regions under study. However, whenever necessary, we use PacBio's or Oxford Nanopore's technologies to obtain longer sequencing reads.

This graph shows the different steps of the AllGenetics' amplicon sequencing service. Therefore it shows a typical workflow of an amplicon sequencing experiment, including DNA isolation, library preparation, high-throughput sequencing, and bioinformatic analysis.

Step 1

We isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.

Step 2

We prepare amplicon libraries for the target genomic region(s). For this, we use universal or in-house-developed primer pairs.

Step 3

We sequence the amplicon libraries using Illumina's MiSeq or NovaSeq technologies.

Step 4

We analyse the data obtained in the previous step to obtain all the variants occurring in your samples.

For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.

What you receive

A report with a summary of the methods followed.

The raw high-throughput sequencing files, which will be delivered through our server, and the corresponding quality control report including demultiplexing and pre-processing (removal of adaptors, primers and chimaeras).

The results of the bioinformatic analysis including clustering of the reads into variants, number of reads obtained per variant, and the generation of rarefaction curves. If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.

Contact us for further information and pricing