Experts in RNA-seq
Differential expression analysis with AllGenetics' RNA-seq service
AllGenetics' RNA-seq service allows us to obtain a large number of reads from the transcriptome which are further analysed using high-throughput bioinformatic methods. The most common application of RNA-seq is to detect differentially expressed genes under given environmental stressors.
Our lab and bioinformatics teams are trained in both bacterial and eukaryote RNA-seq experiments.
Step 1
We isolate RNA from your samples. We have adapted different RNA isolation protocols depending on the starting biological material.
Step 2
We purify the RNA fraction you are interested in (total RNA, mRNA, small RNAs, noncoding RNAs, circular RNAs) and prepare cDNA libraries.
Step 3
We sequence the libraries using the Illumina NovaSeq technology. Millions of reads per library are obtained in this step.
Step 4
We analyse the high-throughput sequencing data obtained in the previous step.
For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.
What you receive
The raw high-throughput sequencing files, which will be delivered through our server, and the corresponding quality control report including demultiplexing and pre-processing.
