Experts in RNA-seq
Differential expression analysis with AllGenetics' RNA-seq service
AllGenetics' RNA-seq service allows us to obtain a large number of reads from the transcriptome which are further analysed using high-throughput bioinformatic methods. The most common application of RNA-seq is to detect differentially expressed genes under given environmental stressors.
Our lab and bioinformatics teams are trained in both bacterial and eukaryote RNA-seq experiments.
We isolate RNA from your samples. We have adapted different RNA isolation protocols depending on the starting biological material.
We purify the RNA fraction you are interested in (total RNA, mRNA, small RNAs, noncoding RNAs, circular RNAs) and prepare cDNA libraries.
We sequence the libraries using the Illumina NovaSeq technology. Millions of reads per library are obtained in this step.
We analyse the high-throughput sequencing data obtained in the previous step.
For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.
What you receive
The raw high-throughput sequencing files, which will be delivered through our server, and the corresponding quality control report including demultiplexing and pre-processing.