Experts in bacterial genome sequencing

Characterise your bacterial strains through our whole genome sequencing service

Characterising bacterial genomes through whole genome sequencing is vitally important in the fields of medicine, ecology, and biotechnology. This is why our bacterial genome sequencing service is more and more in demand by microbiologists from different research backgrounds and with divergent goals.

AllGenetics' expertise on bacterial genomics encompasses either unknown strains and common strains belonging to the Klebsiella, E. coli, Listeria, or Salmonella groups.

We are eager to tailor our protocols to the needs of your research project: therefore, we can take advantage of both short-read (Illumina) or long-read (PacBio) sequencing technologies, and perform on-demand, custom bioinformatic analyses.

This graph shows the different steps of the AllGenetics' bacterial genome sequencing service. Therefore it shows a typical workflow of a bacterial genome sequencing experiment, including DNA isolation, library preparation, high-throughput sequencing, and bioinformatic analysis.

Step 1

We isolate DNA from your samples using specific DNA isolation protocols for bacterial cells.

Step 2

 We prepare genomic libraries using the kits recommended by Illumina.

Step 3

We sequence the libraries using Illumina's MiSeq or NovaSeq technologies. As a norm, we obtain 500 megabases of raw data per library but we can adapt the output to the genome size of the species under study.

Step 4

We analyse the high-throughput sequencing data to obtain your annotated genomes.

What you receive

A report with a summary of the methods followed.
The raw high-throughput sequencing files, which will be delivered through our server, and the corresponding quality control report including demultiplexing and pre-processing.

The results of the bioinformatic analysis including de novo assembly, quality control of the assembly, automated gene prediction and annotation, and average nucleotide identity between the sequenced genomes and publicly available reference genomes. If you require additional analyses, please let us know and we will do our best to meet your needs. For instance, we can carry out plasmid detection, estimates of additional overall genome related indices (AAI, dDDH, and tetranucleotide signature regression), pan-genome analyses, multilocus sequence typing (MLST), and whole-genome phylogenetic analyses.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.

Contact us for further information and pricing