Experts in RNA metabarcoding
Diversity analysis of living organisms in environmental samples with our RNA metabarcoding service
With our RNA metabarcoding service we can identify organisms down to various taxonomic levels and compare the diversity among samples. RNA metabarcoding specifically targets the living fraction of organisms, as RNA rapidly degrades in dead individuals.
After RNA isolation, our lab team will retrotranscribe RNA into cDNA. Then, a workflow similar to that used in DNA metabarcoding experiments will follow.
We isolate RNA from different types of samples such as soil, water, biofilm, sludge, etc.
We prepare RNA metabarcoding libraries for the target taxonomic group(s) -bacteria, fungi, plants, protists, and/or metazoans-. To do so we first retrotranscribe the RNA into cDNA and then use universal or in-house-developed primer pairs.
We sequence the RNA metabarcoding libraries using the Illumina MiSeq or NovaSeq technologies.
We analyse the data obtained in the previous step to determine the taxonomic composition of the samples.
For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.