Experts in bisulphite sequencing
Analyse methylation patterns with AllGenetics' bisulphite sequencing service
AllGenetics' bisulphite sequencing service includes both whole-genome bisulphite sequencing (WGBS) and reduced-representation bisulphite sequencing (RRBS) protocols. While WGBS detects methylated cytosines all throughout the genome, RRBS uses a restriction enzyme to enrich the libraries in CpG contexts, where most of the DNA methylation occurs.
We isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.
We prepare genomic libraries compatible with Illumina's NovaSeq platform. We carry out either WGBS or RRBS protocols in which DNA is treated with bisulphite. In the case of RRBS, a restriction enzyme is used to enrich the libraries in CpG contexts.
We sequence the libraries using the Illumina NovaSeq technology. Millions of reads per library are obtained in this step.
We analyse the high-throughput sequencing data obtained in the previous step.
For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.