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Experts in phage genome sequencing

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Phage characterisation service through whole genome sequencing

With AllGenetics' phage genome sequencing service you will obtain the annotated genomes of your phages. We will purify phage DNA or RNA (either single-stranded or double-stranded) using specific nucleic acid isolation protocols. We will delete the remaining bacterial contamination bioinformatically after sequencing.

With this service you will be able to analyse isolated phages. If your samples contain a viral mix, please consider our metagenomics and metatranscriptomics services.

This graph shows the different steps of the AllGenetics' phage genome sequencing service. Therefore it shows a typical workflow of a phage genome sequencing experiment, including DNA or RNA isolation, library preparation, high-throughput sequencing, and bioinformatic analysis.

Step 1

We isolate DNA or RNA from your purified phage samples using a phage nucleic acid isolation protocol.

Step 2

We prepare genomic libraries using a combination of specific in-house developed protocols and commercial kits suitable for Illumina sequencing.

Step 3

We sequence the libraries using the Illumina MiSeq technology. As a norm, we obtain 5 megabases of raw data per library but we can adapt the output to the genome size of the phage under study.

Step 4

We analyse the high-throughput sequencing data to obtain your annotated genomes.

What you receive

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A report with a summary of the methods followed.
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The raw high-throughput sequencing files, which will be delivered through our server, and the corresponding quality control report including demultiplexing and pre-processing.

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The results of the bioinformatic analysis including removal of bacterial reads, de novo assembly, quality control of the assembly, automated gene prediction and annotation, and average nucleotide identity between the genomes sequenced and publicly available reference genomes. If you require additional analyses, please let us know and we will do our best to meet your needs. For instance, we can carry out pan-genome and whole-genome phylogenetic analyses.
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At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.

Contact us for further information and pricing

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