Experts in metatranscriptomics
Unveiling the living fraction of organisms in a complex sample with our metatranscriptomics service
AllGenetics' metatranscriptomics service provides complete information about the living fraction of organisms in a complex sample. This technique takes advantage of the fact that RNA rapidly degrades in dead individuals. Therefore, it can help elucidate the genes that are being actively transcribed in a certain environment. Metatranscriptomics yields extensive information about functional diversity and operating metabolic pathways and networks.
We can apply metagenomics and metatranscriptomics to a variety of fields, such as microbial ecology, soil biology, ecosystem monitoring, and host-microbe interactions. Remarkably, the metatranscriptomic approach is able to detect ssRNA and dsRNA viruses.
We isolate RNA from different types of samples such as soil, water, biofilm, sludge, etc.
We retrotranscribe the RNA into cDNA and prepare shotgun libraries for all of the biodiversity present in the sample.
We sequence the libraries using the Illumina MiSeq or NovaSeq technologies. We can adapt the output to your specific requirements.
We analyse the high-throughput sequencing data to obtain your annotated metagenomes.
For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.