Experts in DNA metabarcoding

Diversity analysis of environmental samples with our DNA metabarcoding service

With our DNA metabarcoding service we can identify organisms down to various taxonomic levels and compare the diversity among samples. DNA metabarcoding can be used even when DNA is degraded. Therefore, it is possible to analyse species diversity in samples such as soil, faeces, or sediments.

AllGenetics' expertise on DNA metabarcoding encompasses DNA extraction from difficult matrices, custom primer design, or the use of blocking primers to prevent amplification of host DNA. Even though we run most of our projects in Illumina's short read sequencers (MiSeq and NovaSeq), we use PacBio's long-read technology when it becomes necessary to obtain longer barcodes. That way we make sure we get enough taxonomic resolution.

Our bioinformaticians will tailor our pipelines and databases to the specifics of your research project to obtain sound biodiversity estimates from your samples.

This graph shows the different steps of the AllGenetics' DNA metabarcoding service. Therefore it shows a typical workflow of a DNA metabarcoding experiment, including DNA isolation, library preparation, high-throughput sequencing, and bioinformatic analysis.

Step 1

We isolate DNA from different materials such as soil, water, faeces, sediments, organic residues, pollen, etc.

Step 2

We prepare DNA metabarcoding libraries for the target taxonomic group(s) -bacteria, fungi, plants, protists, and/or metazoans-. To do so we use universal or in-house-developed primer pairs. We can also design and use blocking primers during library preparation, if needed.

Step 3

We sequence the DNA metabarcoding libraries using Illumina's MiSeq or NovaSeq technologies.

Step 4

We analyse the data obtained in the previous step to obtain the taxonomic composition of the samples.

For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.

What you receive

A report with a summary of the methods followed.
The raw high-throughput sequencing files, which will be delivered through our server, and the corresponding quality control report including demultiplexing and pre-processing (removal of adaptors, primers and chimaeras)

The results of the bioinformatic analysis including taxon assignment, read count per taxon, and the generation of rarefaction curves. If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.

Contact us for further information and pricing