Experts in MeRIP-seq
Identification of m6A modifications in the RNA with AllGenetics' MeRIP-seq service
With our MeRIP-seq service (also known as m6A-seq) we identify N6-methyladenosine (m6A) modifications in the RNA. These modifications are known to have a large effect on gene expression and mRNA metabolism.
Our lab team immunoprecipitates the RNA containing methylated adenosines by using a specific antibody. We then use the immunoprecipitated RNA for library construction, and we sequence it together with a control RNA sample.
Our bioinformatics team map the reads coming from both the immunoprecipitate sample and the control to a reference transcriptome and compare them to find regions of differential coverage. These regions will point to the location of the m6A residues.
Our full MeRIP-seq service includes RNA fragmentation, RNA immunoprecipitation, library construction of both the immunoprecipitate and the control RNA, high-throughput sequencing, and bioinformatic analyses.
We isolate RNA from your samples. We have adapted different RNA isolation protocols, depending on the starting biological material.
We inmunoprecipitate your samples and prepare cDNA libraries compatible with Illumina’s sequencing platforms.
We sequence the libraries using the Illumina NovaSeq technology. Millions of reads per library are obtained in this step.
We analyse the high-throughput sequencing data obtained in the previous step.
For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.
What you receive
The raw high-throughput sequencing files, which will be delivered through our server, and the corresponding quality control report including demultiplexing and pre-processing.