Experts in MeRIP-seq

Identification of m6A modifications in the RNA with AllGenetics' MeRIP-seq service

With our MeRIP-seq service (also known as m6A-seq) we identify N6-methyladenosine (m6A) modifications in the RNA. These modifications are known to have a large effect on gene expression and mRNA metabolism.

Our lab team immunoprecipitates the RNA containing methylated adenosines by using a specific antibody. We then use the immunoprecipitated RNA for library construction, and we sequence it together with a control RNA sample.

Our bioinformatics team map the reads coming from both the immunoprecipitate sample and the control to a reference transcriptome and compare them to find regions of differential coverage. These regions will point to the location of the m6A residues.

Our full MeRIP-seq service includes RNA fragmentation, RNA immunoprecipitation, library construction of both the immunoprecipitate and the control RNA, high-throughput sequencing, and bioinformatic analyses.

This graph shows the different steps of the AllGenetics' MeRIP-seq service. Therefore it shows a typical workflow of a MeRIP-seq experiment, including RNA isolation, library preparation, high-throughput sequencing, and bioinformatic analysis.

Step 1

We isolate RNA from your samples. We have adapted different RNA isolation protocols, depending on the starting biological material.

Step 2

We inmunoprecipitate your samples and prepare cDNA libraries compatible with Illumina’s sequencing platforms.

Step 3

We sequence the libraries using the Illumina NovaSeq technology. Millions of reads per library are obtained in this step.

Step 4

We analyse the high-throughput sequencing data obtained in the previous step.

For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.

What you receive

A report with a summary of the methods followed.

The raw high-throughput sequencing files, which will be delivered through our server, and the corresponding quality control report including demultiplexing and pre-processing.

The results of the bioinformatic analysis including read alignment against the reference genome, detection of RNA modification sites, and differential modification sites. If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.

Contact us for further information and pricing