Experts in characterising soil and plant microbiome by DNA metabarcoding
Plant and soil microbial diversity characterisation service
Microbial communities play an important role in plant growth and development, being a fundamental part of agrarian soils.
High-throughput sequencing (HTS) technologies have revolutionised the study of microbial communities, allowing the characterisation of species that cannot be detected by culture-dependent methods and making possible the characterisation of all the species present in a given sample, as long as reference sequences are available.
Our soil and plant microbial diversity characterisation service uses DNA metabarcoding, a cost-effective methodology, to screen the bacterial, fungal, protist, and archaea species present in your samples.
Step 1
We isolate DNA from different materials such as soil and plant tissues.
Step 2
We prepare DNA metabarcoding libraries for the target taxonomic group(s) -bacteria, archaea, protists, and/or fungi-. For this we use universal or in-house-developed primer pairs.
Step 3
We sequence the DNA metabarcoding libraries using Illumina's MiSeq or NovaSeq technologies.
Step 4
We analyse the data obtained in the previous step to get the taxonomic composition of the samples.
What you receive
- A list of the Operational Taxonomic Units (OTUs) retrieved per sample.
- The number of sequence reads assigned to each OTU in each sample.
What we need
Your samples which have been appropriately preserved (ethanol, silica gel, frozen, or another suitable preservation method). If required, we provide sampling kits and sampling collection guidelines to ensure that your samples arrive at our lab in optimal conditions.