Experts in characterising vineyard and wine microbiome by DNA metabarcoding

Vineyard and wine microbial diversity characterisation service

Microbial communities are a vital part of agrarian soils, which play an important role in grapevine growth and development, contributing to the terroir. In the last few years, more and more studies link the microbial community structure in vineyards and cellars to variations in the organoleptic properties of wine.

High-throughput sequencing (HTS) technologies have revolutionised how microbial communities are studied, which allows for a precise characterisation of all the species present in a given sample, as long as reference sequences are available. Even those species which cannot be detected by culture-dependent methods can be identified.

Our vineyard and wine microbial diversity characterisation service uses DNA metabarcoding to screen the bacterial, fungal, protist, and archaea species present in your samples, whatever their source might be (soil, plant tissues, must, or wine).

The image shows how the Wine industry can analyse the microbiome associated to grapevine, soil, and vineyard samples. The workflow is composed of DNA isolation from the samples, library preparation, high-throughput sequencing, and bioinformatic analysis. It allows to obtain the microbial taxonomic compositions of the samples analysed.

Step 1

We isolate DNA from your soil or plant samples. We have adapted different DNA isolation protocols, depending on the starting biological material.

Step 2

We prepare DNA metabarcoding libraries for the target taxonomic group(s) -bacteria, archaea, protists, and/or fungi-. For this we use universal or in-house-developed primer pairs.

Step 3

We sequence the DNA metabarcoding libraries using Illumina's MiSeq or NovaSeq technologies.

Section 4

We analyse the data obtained in the previous step to get the taxonomic composition of the samples.

What you receive

A report with a summary of the methods followed.
The raw high-throughput sequencing files
The results of the analysis including:
  • A list of the Operational Taxonomic Units (OTUs) retrieved per sample.
  • The number of sequence reads assigned to each OTU in each sample.
  • A graphic representation of the relative abundances of the different OTUs in each sample.
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery. We offer up to five 60-min sessions per project, depending on the project size.

What we need

Your samples which have been appropriately preserved (ethanol, silica gel, frozen, or another suitable preservation method). If required, we provide sampling kits and sampling collection guidelines to ensure that your samples arrive at our lab in optimal conditions.

Contact us for further information and pricing