Experts in characterising wine yeasts by whole genome sequencing
Characterise your wine yeasts with our whole genome sequencing service
Yeasts, particularly Saccharomyces spp., play a fundamental role in winemaking. Our characterisation service uses whole genome sequencing to differentiate among closely related strains and to screen for relevant genes involved in the fermentative process. This will enable you to identify and select yeast strains with higher fermentation performance, such as higher ethanol and SO2 tolerance, or low acetic acid production and nitrogen requirements.
How we work
Step 1: We isolate DNA from your yeast cultures.
Step 2: We prepare genomic libraries using the kits recommended by Illumina.
Step 3: We sequence the libraries using the Illumina HiSeq or NovaSeq technologies.
Step 4: We analyse the high-throughput sequencing data to obtain your annotated contigs.
What you receive
A species or strain identification certificate.
A report with a summary of the methods followed.
The raw high-throughput sequencing files.
The results of the bioinformatic analysis including:
- Automatic gene annotation.
- Average nucleotide identity between the genomes sequenced and other yeast genomes available in reference databases.
We can also carry out on-demand bioinformatic analyses, which can be applied to investigate strain relatedness or look for specific gene sets.
At no additional cost, our project specialists will be available to you after project delivery. We offer up to five 60-min sessions per project, depending on the project size.
What we need
Your yeast cultures which have been appropriately preserved (fresh, frozen, or lyophilised). Please contact us if you need assistance on how to preserve and ship your cultures.