Experts in amplicon sequencing
Analyse all the gene variants with our amplicon sequencing service
At AllGenetics, we can sequence all the gene variants of heterogeneous multi-copy regions. With our amplicon sequencing service you will be able to recover all the diversity regardless of the number of indels or point mutations. This is extremely useful when studying gene families such as the vertebrate major histocompatibility complex (MHC) or the ribosomal non-transcribed spacers (ITS1 and ITS2).
Step 1: We isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.
Step 2: We prepare amplicon libraries for the target genomic region(s). For this, we use universal or in-house-developed primer pairs.
Step 3: We sequence the amplicon libraries using the Illumina MiSeq technology.
Step 4: We analyse the data obtained in the previous step to obtain all the variants occurring in your samples.
For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.
What you receive
A report with a summary of the methods followed.
The raw high-throughput sequencing files, which will be delivered through our server.
The results of the bioinformatic analysis including demultiplexing, pre-processing and quality control of the raw data, chimaera removal, clustering of the reads into variants, number of reads obtained per variant, and the generation of rarefaction curves.
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.