Experts in bacterial genome sequencing
Characterise your bacterial strains through our whole genome sequencing service
Characterising bacterial genomes through whole genome sequencing is vitally important in the fields of medicine, ecology, and biotechnology. This is why our sequencing service is more and more in demand by microbiologists from different research backgrounds and with divergent goals.
Step 1: We isolate DNA from your samples using specific DNA isolation protocols for bacterial cells.
Step 2: We prepare genomic libraries using the kits recommended by Illumina.
Step 3: We sequence the libraries using the Illumina MiSeq technology. As a norm, we obtain 500 megabases of raw data per library but we can adapt the output to the genome size of the species under study.
Step 4: We analyse the high-throughput sequencing data to obtain your annotated genomes.
What you receive
A report with a summary of the methods followed.
The raw high-throughput sequencing files, which will be delivered through our server.
The results of the bioinformatic analysis including demultiplexing, pre-processing and quality control of the raw data, de novo assembly, removal of duplicate reads, automatic gene annotation, and average nucleotide identity between the genomes sequenced and publicly available reference genomes.
If you require additional analyses, please let us know and we will do our best to meet your needs. For instance, we can carry out plasmid detection, estimates of additional overall genome related indices (AAI, dDDH, and tetranucleotide signature regression), pan-genome analyses, multilocus sequence typing (MLST), and whole-genome phylogenetic analyses.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.