Experts in bisulphite sequencing
Analyse methylation patterns with AllGenetics' bisulphite sequencing service
Our service includes both whole-genome bisulphite sequencing (WGBS) and reduced-representation bisulphite sequencing (RRBS) protocols. While WGBS detects methylated cytosines all throughout the genome, RRBS uses a restriction enzyme to enrich the libraries in CpG contexts, where most of the DNA methylation occurs.
Step 1: We isolate DNA from your samples. We have adapted different DNA isolation protocols, depending on the starting biological material.
Step 2: We prepare genomic libraries compatible with Illumina’s HiSeq platforms. We carry out either WGBS or RRBS protocols in which DNA is treated with bisulphite. In the case of RRBS, a restriction enzyme is used to enrich the libraries in CpG contexts.
Step 3: We sequence the libraries using the Illumina HiSeq or NovaSeq technologies. Millions of reads per library are obtained in this step.
Step 4: We analyse the high-throughput sequencing data obtained in the previous step.
For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.
What you receive
A report with a summary of the methods followed.
The raw high-throughput sequencing files, which will be delivered through our server.
The results of the bioinformatic analysis including demultiplexing, pre-processing and quality control of the raw data, bisulphite-sensitive read alignment against the reference genome, removal of duplicate reads (WGBS) or quality filtering by restriction sites (RRBS), methylation calling, and detection of differentially methylated positions (DMPs) and regions (DMRs) among conditions.
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.