Experts in DNA metabarcoding
Diversity analysis of environmental samples with our DNA metabarcoding service
With our DNA metabarcoding service we can identify organisms down to various taxonomic levels and compare the diversity among samples. DNA metabarcoding can be used even when DNA is degraded. Therefore, it is possible to analyse species diversity in samples such as soil, faeces, or sediments.
Step 1: We isolate DNA from different materials such as soil, water, faeces, sediments, organic residues, pollen, etc.
Step 2: We prepare DNA metabarcoding libraries for the target taxonomic group(s) -bacteria, fungi, plants, protists, and/or metazoans-. To do so we use universal or in-house-developed primer pairs. We can also design and use blocking primers during library preparation, if needed.
Step 3: We sequence the DNA metabarcoding libraries using the Illumina MiSeq technology.
Step 4: We analyse the data obtained in the previous step to obtain the taxonomic composition of the samples.
For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.
What you receive
A report with a summary of the methods followed.
The raw high-throughput sequencing files, which will be delivered through our server.
The results of the bioinformatic analysis including demultiplexing, pre-processing and quality control of the raw data, chimaera removal, taxon assignment, read count per taxon, and the generation of rarefaction curves.
If you require additional analyses, please let us know and we will do our best to meet your needs.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.