Experts in MeRIP-seq

Identification of m6A modifications in the RNA with AllGenetics' MeRIP-seq service

MeRIP-seq (also known as m6A-seq) is a technique that is capable of identifying N6-methyladenosine (m6A) modifications in the RNA. This modification is known to have a large effect on gene expression and mRNA metabolism.

The technique is based on the inmunoprecipitiation of RNA containing methylated adenosines by using a specific antibody. The inmunoprecipitated RNA is then used for library construction, and sequenced together with a control RNA sample. Reads coming from the inmunoprecipitated sample and the control are mapped to a reference trascriptome and compared to find regions of differential coverage, which will point to the location of the m6A residues.

Our full service includes RNA fragmentation, RNA inmunoprecipitation, library construction of both the inmunoprecipitated and the control RNA, high-throughput sequencing, and bioinformatic analyses.

RNA-seq service - AllGenetics

Step 1: We isolate RNA from your samples. We have adapted different RNA isolation protocols, depending on the starting biological material.

Step 2: We inmunoprecipitate your samples and prepare cDNA libraries compatible with Illumina’s sequencing platforms.

Step 3: We sequence the libraries using the Illumina NovaSeq technology. Millions of reads per library are obtained in this step.

Step 4: We analyse the high-throughput sequencing data obtained in the previous step.

For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.

What you receive

A report with a summary of the methods followed.

The raw high-throughput sequencing files, which will be delivered through our server, and the corresponding quality control report including demultiplexing and pre-processing.

The results of the bioinformatic analysis including read alignment against the reference genome, detection of RNA modification sites, and differential modification sites.

If you require additional analyses, please let us know and we will do our best to meet your needs.

At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size. 

Contact us for further information and pricing >

- Genomics for researchers

+ Genomic solutions for your company

+ Bioinformatics

+ Training

+ Genomic consulting

Genomics for researchers