Experts in phage genome sequencing
Phage characterisation service through whole genome sequencing
Our phage characterisation service through whole genome sequencing uses high-throughput sequencing to obtain the annotated genomes of your phages. Phage DNA is purified using specific DNA isolation protocols and the remaining bacterial DNA contamination is deleted bioinformatically after sequencing.
Step 1: We isolate DNA from your samples using a phage DNA isolation protocol.
Step 2: We prepare genomic libraries using the kits recommended by Illumina.
Step 3: We sequence the libraries using the Illumina MiSeq technology. As a norm, we obtain 5 megabases of raw data per library but we can adapt the output to the genome size of the phage under study.
Step 4: We analyse the high-throughput sequencing data to obtain your annotated genomes.
What you receive
A report with a summary of the methods followed.
The raw high-throughput sequencing files, which will be delivered through our server.
The results of the bioinformatic analysis including demultiplexing, pre-processing and quality control of the raw data, removal of bacterial reads, de novo assembly, removal of duplicate reads, automatic gene annotation, and average nucleotide identity between the genomes sequenced and publicly available reference genomes.
If you require additional analyses, please let us know and we will do our best to meet your needs. For instance, we can carry out pan-genome and whole-genome phylogenetic analyses.
At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.