Experts in RNA metabarcoding

Diversity analysis of living organisms in environmental samples

With our RNA metabarcoding service we can identify organisms down to various taxonomic levels and compare the diversity among samples. This analysis specifically targets the living fraction of organisms, as RNA rapidly degrades in dead individuals.

RNA metabarcoding service - AllGenetics

Step 1: We isolate RNA from different types of samples such as soil, water, biofilm, sludge, etc.

Step 2: We prepare RNA metabarcoding libraries for the target taxonomic group(s) -bacteria, fungi, plants, protists, and/or metazoans-. To do so we first retrotranscribe the RNA into cDNA and then use universal or in-house-developed primer pairs.

Step 3: We sequence the RNA metabarcoding libraries using the Illumina MiSeq or NovaSeq technologies.

Step 4: We analyse the data obtained in the previous step to determine the taxonomic composition of the samples.

For your convenience, we can carry out the entire project or only those steps you require. As a norm, steps 2 and 3 should be ordered jointly.

What you receive

A report with a summary of the methods followed.

The raw high-throughput sequencing files, which will be delivered through our server.

The results of the bioinformatic analysis including demultiplexing, quality control of the raw data and pre-processing (removal of adaptors, primers, and chimaeras), taxon assignment, read count per taxon, and the generation of rarefaction curves.

If you require additional analyses, please let us know and we will do our best to meet your needs.

At no additional cost, our project specialists will be available to you after project delivery and during the editorial process of your scientific articles. We offer up to five 60-min sessions per project, depending on the project size.

Contact us for further information and pricing >

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