Introduction to high-throughput sequencing: Illumina, PacBio, and Oxford Nanopore
In this 7-hour course, we will cover the basics of the different sequencing chemistries and platforms that are now available in the market, with a focus on the Illumina technology. During the course we will review how high-throughput sequencing has revolutionised our understanding of biology and has greatly accelerated research in many areas of the life sciences.
The course will also teach some of the main applications in the domain of life science research, covering the main details of a high-throughput sequencing experiment, and focusing on the technical aspects of DNA or RNA isolation, library preparation, and sequencing. Lastly, we will also introduce the key aspects to determine the quality of the data generated with high-throughput sequencing.
By the end of the course, participants will be able to understand how high-throughput sequencing technique works and how it can be applied to their current or future research.
Language: Spanish and English.
Number of places available: 30.
Instructors: Antón Vizcaíno and Dr Belén Carro.
Target audience and requirements
Master's students, early career researchers (pre-docs and post-docs), or senior researchers, either from Industry or Academia, with zero or little prior contact with high-throughput sequencing technologies. Participants will be expected to be familiar with the most common molecular biology techniques.
Although this course does not include any data analysis, participants are welcome to bring their own laptops.
An attendance certificate will be provided at the end of the course.